ddRAD-seq of the alpine bamboos - SRA

SRX5087662: ddRAD-seq of the alpine bamboos
1 ILLUMINA (HiSeq X Ten) run: 8.5M spots, 1.2G bases, 475.6Mb downloads

Design: Cleaned DNA was double-digested with AvaII and MspI restriction enzymes followed by adapter ligation with P1 (DNA barcodes adapter) and P2 (varied in length). Ligated fragments were then pooled and loaded on 2.0% agarose gel for size selection (600-700bp). Recovered DNA fragments were then used as templates for PCR amplification with 16 cycles and products were purified in spin columns (E.Z.N.A.? Cycle Pure Kit provides). Finally the library was diluted to 10nM and sequenced on the Illumina HiSeq X Ten System (San Diego CA USA)

Submitted by: Kunming Institute of Botany

Study: The evolution of the Hengduan Mountains biodiversity hotspot revealed by the origin and rapid diversification of the alpine bamboos

PRJNA505605 • SRP172004 • All experiments • All runs

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Using the alpine bamboos, which are primarily distributed in this region, as a model system to explore the evolution of the Hengduan Mountains biodiversity hotspot .

Sample:

SAMN10439817 • SRS4099765 • All experiments • All runs

Organism: Oldeania humbertii

Library:

Name: GC_4_L2_032

Instrument: HiSeq X Ten

Strategy: RAD-Seq

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Runs: 1 run, 8.5M spots, 1.2G bases, 475.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR8270878	8,512,439	1.2G	475.6Mb	2019-01-31

ID:: 6864085

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